SNPs by AFLP (SBA): a rapid SNP isolation strategy for non-model organisms | Zendy

JeanChristophe Nicod | Zendy

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SNPs by AFLP (SBA): a rapid SNP isolation strategy for non-model organisms

Author(s) -

JeanChristophe Nicod

Publication year - 2003

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gng019

Subject(s) - biology , single nucleotide polymorphism , genetics , snp , amplified fragment length polymorphism , molecular inversion probe , salmo , dna sequencing , population , snp genotyping , brown trout , computational biology , genomic dna , model organism , dna , genotype , gene , genetic diversity , fish <actinopterygii> , fishery , demography , sociology

Despite the great potential of single nucleotide polymorphism (SNP) markers in evolutionary studies, in particular for inferring population genetic parameters, SNP analysis has almost exclusively been limited to humans and 'genomic model' organisms, due to the lack of available sequence data in non-model organisms. Here, we describe a rapid and cost effective method to isolate candidate SNPs in non-model organisms. This SNP isolation strategy consists basically in the direct sequencing of amplified fragment length polymorphism bands. In a first application of this method, 10 unique DNA fragments that contained 24 SNPs were discovered in 11.11 kb of sequenced genomic DNA of a non-model species, the brown trout (Salmo trutta).

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