Polyamine-assisted rapid and clean cleavage of oligonucleotides from cis-diol bearing universal support | Zendy

Pravesh Kumar | Zendy

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Polyamine-assisted rapid and clean cleavage of oligonucleotides from cis-diol bearing universal support

Author(s) -

Pravesh Kumar

Publication year - 2002

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gnf130

Subject(s) - synthon , oligonucleotide , polyamine , biology , phosphoramidite , cleavage (geology) , combinatorial chemistry , diol , biochemistry , dna , stereochemistry , organic chemistry , chemistry , paleontology , fracture (geology)

Polyamine-assisted deprotection conditions have been developed for the rapid and clean cleavage of oligonucleotide chains from a cis-diol group bearing universal polymer support, making it compatible with modern oligonucleotide synthesis via all types of phosphoramidite synthons, including base labile protecting group bearing synthons as well. The synthesized oligonucleotides were found to be comparable with the corresponding standard oligomers with respect to their retention time on HPLC, mass on MALDI-TOF and biological activity in PCR amplification.

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