Sequence tagged microsatellite profiling (STMP): improved isolation of DNA sequence flanking target SSRs | Zendy

Matthew Hayden | Zendy; G Good | Zendy; P. J. Sharp | Zendy

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Sequence tagged microsatellite profiling (STMP): improved isolation of DNA sequence flanking target SSRs

Author(s) -

Matthew Hayden,

G Good,

P. J. Sharp

Publication year - 2002

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gnf129

Subject(s) - microsatellite , biology , primer (cosmetics) , genetics , dna sequencing , dna , sequence tagged site , sequence (biology) , polymerase chain reaction , computational biology , sequence analysis , gene , gene mapping , allele , chemistry , organic chemistry , chromosome

Sequence tagged microsatellite profiling (STMP) enables the rapid development of large numbers of co-dominant DNA markers, known as sequence tagged microsatellites (STMs). Each STM is amplified by PCR using a single primer specific to the conserved DNA sequence flanking the microsatellite repeat in combination with a universal primer that anchors to the 5'-ends of the microsatellites. It is also possible to convert STMs into conventional microsatellite, or simple sequence repeat (SSR), markers that are amplified using a pair of primers flanking the repeat sequence. Here, we describe a modification of the STMP procedure to significantly improve the capacity to convert STMs into conventional SSRs and, therefore, facilitate the development of highly specific DNA markers for purposes such as marker-assisted breeding. The usefulness of this technique was demonstrated in bread wheat.M. J. Hayden, G. Good and P. J. Shar

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