Use of hybridization kinetics for differentiating specific from non-specific binding to oligonucleotide microarrays | Zendy

H. Dai | Zendy

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Use of hybridization kinetics for differentiating specific from non-specific binding to oligonucleotide microarrays

Author(s) -

H. Dai

Publication year - 2002

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gnf085

Subject(s) - biology , oligonucleotide , dna microarray , kinetics , receptor–ligand kinetics , false positive paradox , microbiology and biotechnology , computational biology , genetics , gene , gene expression , physics , receptor , quantum mechanics , machine learning , computer science

Hybridization kinetics were found to be significantly different for specific and non-specific binding of labeled cRNA to surface-bound oligonucleotides on microarrays. We show direct evidence that in a complex sample specific binding takes longer to reach hybridization equilibrium than the non- specific binding. We find that this property can be used to estimate and to correct for the hybridization contributed by non-specific binding. Useful applications are illustrated including the selection of superior oligonucleotides, and the reduction of false positives in exon identification.

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