An efficient haplotyping method with DNA pools | Zendy

Efraim Inbar | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

An efficient haplotyping method with DNA pools

Author(s) -

Efraim Inbar

Publication year - 2002

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gnf075

Subject(s) - haplotype , biology , linkage disequilibrium , genetics , tag snp , haplotype estimation , single nucleotide polymorphism , genotyping , snp genotyping , allele frequency , allele , snp , genetic association , genetic marker , genotype , gene

Determination of haplotype frequencies (the joint distribution of genetic markers) in large population samples is a powerful tool for association studies. This is due to their greater extent of polymorphism since any two bi-allelic single nucleotide polymorphisms (SNPs) generate a potential four-allele genetic marker. Therefore, a haplotype may capture a given functional polymorphism with higher statistical power than its SNP components. The statistical estimation of haplotype frequencies, usually employed in linkage disequilibrium studies, requires individual genotyping for each SNP in the haplotype, thus making it an expensive process. In this study, we describe a new method for direct measurement of haplotype frequencies in DNA pools by allele-specific, long-range haplotype amplification. The proposed method allows the efficient determination of haplotypes composed of two SNPs in close vicinity (up to 20 kb).

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research