Physical and functional interaction between SET1/COMPASS complex component CFP-1 and a Sin3S HDAC complex in C. elegans | Zendy

Flore Beurton | Zendy; Przemysław Stempor | Zendy; Matthieu Caron | Zendy; Alex Appert | Zendy; Dong Yan | Zendy; Ron A.-J. Chen | Zendy; David Cluet | Zendy; Yohann Couté | Zendy; Marion Herbette | Zendy; Ni Huang | Zendy; Hélène Polvèche | Zendy; Martin Spichty | Zendy; Cécile Bedet | Zendy; Julie Ahringer | Zendy; Francesca Palladino | Zendy

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Physical and functional interaction between SET1/COMPASS complex component CFP-1 and a Sin3S HDAC complex in C. elegans

Author(s) -

Flore Beurton,

Przemysław Stempor,

Matthieu Caron,

Alex Appert,

Dong Yan,

Ron A.-J. Chen,

David Cluet,

Yohann Couté,

Marion Herbette,

Ni Huang,

Hélène Polvèche,

Martin Spichty,

Cécile Bedet,

Julie Ahringer,

Francesca Palladino

Publication year - 2019

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkz880

Subject(s) - biology , compass , component (thermodynamics) , caenorhabditis elegans , genetics , computational biology , microbiology and biotechnology , evolutionary biology , gene , cartography , geography , physics , thermodynamics

The CFP1 CXXC zinc finger protein targets the SET1/COMPASS complex to non-methylated CpG rich promoters to implement tri-methylation of histone H3 Lys4 (H3K4me3). Although H3K4me3 is widely associated with gene expression, the effects of CFP1 loss vary, suggesting additional chromatin factors contribute to context dependent effects. Using a proteomics approach, we identified CFP1 associated proteins and an unexpected direct link between Caenorhabditis elegans CFP-1 and an Rpd3/Sin3 small (SIN3S) histone deacetylase complex. Supporting a functional connection, we find that mutants of COMPASS and SIN3 complex components genetically interact and have similar phenotypic defects including misregulation of common genes. CFP-1 directly binds SIN-3 through a region including the conserved PAH1 domain and recruits SIN-3 and the HDA-1/HDAC subunit to H3K4me3 enriched promoters. Our results reveal a novel role for CFP-1 in mediating interaction between SET1/COMPASS and a Sin3S HDAC complex at promoters.

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