Open AccessOptimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1
Author(s) -
Jose R. Quejada,
Seon-Hye E. Park,
Daniel W. Awari,
Fan Shi,
Hannah E. Yamamoto,
Fuun Kawano,
Juergen C. Jung,
Masayuki Yazawa
Publication year - 2017
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/gkx804
Subject(s) - biology , cryptochrome , transcription factor , transcription (linguistics) , optogenetics , arabidopsis , microbiology and biotechnology , computational biology , genetics , gene , circadian clock , mutant , linguistics , philosophy , neuroscience
Light-inducible systems allow spatiotemporal control of a variety of biological activities. Here, we report newly optimized optogenetic tools to induce transcription with light in mammalian cells, using the Arabidopsis photoreceptor Flavin Kelch-repeat F-box 1 (FKF1) and its binding partner GIGANTEA (GI) as well as CRY2/CIB1. By combining the mutagenesis of FKF1 with the optimization of a split FKF1/GI dimerized Gal4-VP16 transcriptional system, we identified constructs enabling significantly improved light-triggered transcriptional induction. In addition, we have improved the CRY2/CIB1-based light-inducible transcription with split construct optimization. The improvements regarding the FKF1/GI- and CRY2/CIB1-based systems will be widely applicable for the light-dependent control of transcription in mammalian cells.
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