Orientation-dependent FRET system reveals differences in structures and flexibilities of nicked and gapped DNA duplexes | Zendy

Hiromu Kashida | Zendy; Ayako Kurihara | Zendy; Hayato Kawai | Zendy; Hiroyuki Asanuma | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Orientation-dependent FRET system reveals differences in structures and flexibilities of nicked and gapped DNA duplexes

Author(s) -

Hiromu Kashida,

Ayako Kurihara,

Hayato Kawai,

Hiroyuki Asanuma

Publication year - 2017

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkx200

Subject(s) - förster resonance energy transfer , dna , biology , duplex (building) , biophysics , single molecule fret , molecule , crystallography , genetics , fluorescence , physics , chemistry , quantum mechanics

Differences in structures and flexibilities of DNA duplexes play important roles on recognition by DNA-binding proteins. We herein describe a novel method for structural analyses of DNA duplexes by using orientation dependence of Förster resonance energy transfer (FRET). We first analyzed canonical B-form duplex and correct structural parameters were obtained. The experimental FRET efficiencies were in excellent agreement with values theoretically calculated by using determined parameters. We then investigated DNA duplexes with nick and gaps, which are key intermediates in DNA repair systems. Effects of gap size on structures and flexibilities were successfully revealed. Since our method is facile and sensitive, it could be widely used to analyze DNA structures containing damages and non-natural molecules.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research