Nucleic Acids Research | Zendy

Michael  Evans | Zendy; Andrew  Malvern | Zendy; Kate  Puttick | Zendy

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Nucleic Acids Research

Author(s) -

Michael Evans,

Andrew Malvern,

Kate Puttick

Publication year - 2015

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkv852

Subject(s) - biology , nucleic acid , computational biology , biochemistry

The 5'-termini were precisely mapped for five constitutive and one UV-inducible transcript from the Sulfolobus virus-like particle SSV1. The comparison of the DNA sequences around these transcriptional initiation sites revealed the presence of two conserved sequence elements: a trinucleotide sequence close to the initiation site itself and an AT-rich hexanucleotide sequence centered about 26 nucleotides upstream of it. Similar DNA sequences were found upstream of the transcriptional start sites for the ribosomal RNA genes in Sulfolobus and upstream of transcriptional start sites in other archaebacteria, allowing the derivation of a general consensus sequence for archaebacteriai promoters. This consensus sequence is unlike that found in eubacteria but it resembles promoters recognized by eukaryotic RNA polymerase I

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