Simultaneous live imaging of the transcription and nuclear position of specific genes | Zendy

Hiroshi Ochiai | Zendy; Takeshi Sugawara | Zendy; Takashi Yamamoto | Zendy

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Simultaneous live imaging of the transcription and nuclear position of specific genes

Author(s) -

Hiroshi Ochiai,

Takeshi Sugawara,

Takashi Yamamoto

Publication year - 2015

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkv624

Subject(s) - biology , homeobox protein nanog , transactivation , genome , gene , gene expression , genetics , transcription factor , nuclear gene , regulation of gene expression , transcription (linguistics) , computational biology , live cell imaging , embryonic stem cell , microbiology and biotechnology , induced pluripotent stem cell , cell , linguistics , philosophy

The relationship between genome organization and gene expression has recently been established. However, the relationships between spatial organization, dynamics, and transcriptional regulation of the genome remain unknown. In this study, we developed a live-imaging method for simultaneous measurements of the transcriptional activity and nuclear position of endogenous genes, which we termed the ‘Real-time Observation of Localization and EXpression (ROLEX)’ system. We demonstrated that ROLEX is highly specific and does not affect the expression level of the target gene. ROLEX enabled detection of sub-genome-wide mobility changes that depended on the state of Nanog transactivation in embryonic stem cells. We believe that the ROLEX system will become a powerful tool for exploring the relationship between transcription and nuclear dynamics in living cells.

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