Stable complex formation of CENP-B with the CENP-A nucleosome | Zendy

Risa Fujita | Zendy; Koichiro Otake | Zendy; Yasuhiro Arimura | Zendy; Naoki Horikoshi | Zendy; Yuta Miya | Zendy; Tatsuya Shiga | Zendy; Akihisa Osakabe | Zendy; Hiroaki Tachiwana | Zendy; Junichirou Ohzeki | Zendy; Vladimir Larionov | Zendy; Hiroshi Masumoto | Zendy; Hitoshi Kurumizaka | Zendy

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Stable complex formation of CENP-B with the CENP-A nucleosome

Author(s) -

Risa Fujita,

Koichiro Otake,

Yasuhiro Arimura,

Naoki Horikoshi,

Yuta Miya,

Tatsuya Shiga,

Akihisa Osakabe,

Hiroaki Tachiwana,

Junichirou Ohzeki,

Vladimir Larionov,

Hiroshi Masumoto,

Hitoshi Kurumizaka

Publication year - 2015

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkv405

Subject(s) - nucleosome , biology , linker dna , chromatosome , chromatin , histone h3 , histone , dna , centromere , genetics , microbiology and biotechnology , biophysics , chromosome , gene

CENP-A and CENP-B are major components of centromeric chromatin. CENP-A is the histone H3 variant, which forms the centromere-specific nucleosome. CENP-B specifically binds to the CENP-B box DNA sequence on the centromere-specific repetitive DNA. In the present study, we found that the CENP-A nucleosome more stably retains human CENP-B than the H3.1 nucleosome in vitro. Specifically, CENP-B forms a stable complex with the CENP-A nucleosome, when the CENP-B box sequence is located at the proximal edge of the nucleosome. Surprisingly, the CENP-B binding was weaker when the CENP-B box sequence was located in the distal linker region of the nucleosome. This difference in CENP-B binding, depending on the CENP-B box location, was not observed with the H3.1 nucleosome. Consistently, we found that the DNA-binding domain of CENP-B specifically interacted with the CENP-A-H4 complex, but not with the H3.1-H4 complex, in vitro. These results suggested that CENP-B forms a more stable complex with the CENP-A nucleosome through specific interactions with CENP-A, if the CENP-B box is located proximal to the CENP-A nucleosome. Our in vivo assay also revealed that CENP-B binding in the vicinity of the CENP-A nucleosome substantially stabilizes the CENP-A nucleosome on alphoid DNA in human cells.

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