Identification of a Mg2+-sensitive ORF in the 5′-leader of TRPM7 magnesium channel mRNA | Zendy

Inna A. Nikonorova | Zendy; Nikolay V. Kornakov | Zendy; Sergey E. Dmitriev | Zendy; Konstantin S. Vassilenko | Zendy; Alexey G. Ryazanov | Zendy

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Identification of a Mg2+-sensitive ORF in the 5′-leader of TRPM7 magnesium channel mRNA

Author(s) -

Inna A. Nikonorova,

Nikolay V. Kornakov,

Sergey E. Dmitriev,

Konstantin S. Vassilenko,

Alexey G. Ryazanov

Publication year - 2014

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gku951

Subject(s) - trpm7 , biology , open reading frame , upstream open reading frame , magnesium , messenger rna , translation (biology) , microbiology and biotechnology , gene , genetics , peptide sequence , ion channel , chemistry , receptor , organic chemistry

TRPM7 is an essential and ubiquitous channel-kinase regulating cellular influx of Mg2+. Although TRPM7 mRNA is highly abundant, very small amount of the protein is detected in cells, suggesting post-transcriptional regulation of trpm7 gene expression. We found that TRPM7 mRNA 5'-leader contains two evolutionarily conserved upstream open reading frames that act together to drastically inhibit translation of the TRPM7 reading frame at high magnesium levels and ensure its optimal translation at low magnesium levels, when the activity of the channel-kinase is most required. The study provides the first example of magnesium channel synthesis being controlled by Mg2+ in higher eukaryotes.

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