Whole-body scanning PCR; a highly sensitive method to study the biodistribution of mRNAs, noncoding RNAs and therapeutic oligonucleotides | Zendy

Julien A. Boos | Zendy; David W. Kirk | Zendy; Mari-Luz Piccolotto | Zendy; Werner Zuercher | Zendy; Sandro Gfeller | Zendy; Philippe Neuner | Zendy; Andre Dattler | Zendy; William Wishart | Zendy; Fabian Von Arx | Zendy; Michael B. Beverly | Zendy; Jesper Kammersgaard Christensen | Zendy; Karine Litherland | Zendy; Esther van de Kerkhof | Zendy; Pieter Swart | Zendy; Thomas Faller | Zendy; Armin Beyerbach | Zendy; Dylan Morrissey | Zendy; Juerg Hunziker | Zendy; Iwan Beuvink | Zendy

Open Access

Whole-body scanning PCR; a highly sensitive method to study the biodistribution of mRNAs, noncoding RNAs and therapeutic oligonucleotides

Author(s) -

Julien A. Boos,

David W. Kirk,

Mari-Luz Piccolotto,

Werner Zuercher,

Sandro Gfeller,

Philippe Neuner,

Andre Dattler,

William Wishart,

Fabian Von Arx,

Michael B. Beverly,

Jesper Kammersgaard Christensen,

Karine Litherland,

Esther van de Kerkhof,

Pieter Swart,

Thomas Faller,

Armin Beyerbach,

Dylan Morrissey,

Juerg Hunziker,

Iwan Beuvink

Publication year - 2013

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkt515

Subject(s) - oligonucleotide , biology , biodistribution , aptamer , rna , computational biology , microbiology and biotechnology , nucleic acid , dna , in vivo , biochemistry , gene , genetics

Efficient tissue-specific delivery is a crucial factor in the successful development of therapeutic oligonucleotides. Screening for novel delivery methods with unique tissue-homing properties requires a rapid, sensitive, flexible and unbiased technique able to visualize the in vivo biodistribution of these oligonucleotides. Here, we present whole body scanning PCR, a platform that relies on the local extraction of tissues from a mouse whole body section followed by the conversion of target-specific qPCR signals into an image. This platform was designed to be compatible with a novel RT-qPCR assay for the detection of siRNAs and with an assay suitable for the detection of heavily chemically modified oligonucleotides, which we termed Chemical-Ligation qPCR (CL-qPCR). In addition to this, the platform can also be used to investigate the global expression of endogenous mRNAs and non-coding RNAs. Incorporation of other detection systems, such as aptamers, could even further expand the use of this technology.

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