Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system | Zendy

Kei Fujiwara | Zendy; Tsutomu Katayama | Zendy; Shinichiro M. Nomura | Zendy

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Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system

Author(s) -

Kei Fujiwara,

Tsutomu Katayama,

Shinichiro M. Nomura

Publication year - 2013

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkt489

Subject(s) - biology , control of chromosome duplication , dna replication , primase , replication factor c , replication protein a , origin recognition complex , eukaryotic dna replication , pre replication complex , helicase , prokaryotic dna replication , dna clamp , dna polymerase ii , microbiology and biotechnology , transcription (linguistics) , dna , dna polymerase , genetics , minichromosome maintenance , gene , rna , transcription factor , dna binding protein , reverse transcriptase , linguistics , philosophy

Replication of all living cells relies on the multirounds flow of the central dogma. Especially, expression of DNA replication proteins is a key step to circulate the processes of the central dogma. Here we achieved the entire sequential transcription-translation-replication process by autonomous expression of chromosomal DNA replication machineries from a reconstituted transcription-translation system (PURE system). We found that low temperature is essential to express a complex protein, DNA polymerase III, in a single tube using the PURE system. Addition of the 13 genes, encoding initiator, DNA helicase, helicase loader, RNA primase and DNA polymerase III to the PURE system gave rise to a DNA replication system by a coupling manner. An artificial genetic circuit demonstrated that the DNA produced as a result of the replication is able to provide genetic information for proteins, indicating the in vitro central dogma can sequentially undergo two rounds.

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