Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs | Zendy

James A Casbon | Zendy; Andrew F. Slatter | Zendy; E. Musgrave-Brown | Zendy; Robert J. Osborne | Zendy; Conrad Lichtenstein | Zendy; Sydney Brenner | Zendy

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Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs

Author(s) -

James A Casbon,

Andrew F. Slatter,

E. Musgrave-Brown,

Robert J. Osborne,

Conrad Lichtenstein,

Sydney Brenner

Publication year - 2013

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkt228

Subject(s) - biology , polymerase chain reaction , primer (cosmetics) , primer extension , genetics , intramolecular force , dna , inverse polymerase chain reaction , population , computational biology , multiplex polymerase chain reaction , base sequence , gene , stereochemistry , demography , sociology , chemistry , organic chemistry

We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small number of primer extension steps to rapidly enable uniform sequence coverage of long contiguous sequence targets in large numbers of samples at low cost on desktop next-generation sequencers.

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