Open AccessNucleic Acids Research: VOLUME 38 ISSUE 17 2010
Author(s) -
J. K. Elder,
D. K. Green,
E. M. Southern
Publication year - 2010
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/gkq877
Subject(s) - biology , nucleic acid , volume (thermodynamics) , computational biology , biochemistry , thermodynamics , physics
We have developed a large format digital scanner for several applications in nucleic acid analysis. Here we describe the scanning of autoradiographs of DHA sequencing gels and a set of programs for reading the base sequence. The programs correct distortions in the gel, recognize bands by their characteristic shape and assign bases to bands by weighting band position and intensity. The sequence read in this way is as accurate as that read by an expert.
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