DeepSAGE—digital transcriptomics with high sensitivity, simple experimental protocol and multiplexing of samples | Zendy

Kåre Lehmann Nielsen | Zendy; Annabeth Laursen Høgh | Zendy; Jeppe Emmersen | Zendy

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DeepSAGE—digital transcriptomics with high sensitivity, simple experimental protocol and multiplexing of samples

Author(s) -

Kåre Lehmann Nielsen,

Annabeth Laursen Høgh,

Jeppe Emmersen

Publication year - 2006

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkl714

Subject(s) - biology , transcriptome , gene expression profiling , gene , gene expression , computational biology , multiplexing , digital polymerase chain reaction , abiotic stress , dna sequencing , genetics , polymerase chain reaction , computer science , telecommunications

Digital transcriptomics with pyrophosphatase based ultra-high throughput DNA sequencing of di-tags provides high sensitivity and cost-effective gene expression profiling. Sample preparation and handling are greatly simplified compared to Serial Analysis of Gene Expression (SAGE). We compare DeepSAGE and LongSAGE data and demonstrate greater power of detection and multiplexing of samples derived from potato. The transcript anal- ysis revealed a great abundance of up-regulated potato transcripts associated with stress in dormant potatoes compared to harvest. Importantly, many transcripts were detected that cannot be matched to known genes, but is likely to be part of the abiotic stress-response in potato.

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