Identification of miRNA targets with stable isotope labeling by amino acids in cell culture | Zendy

Jeppe Vinther | Zendy; Mads M. Hedegaard | Zendy; Paul P. Gardner | Zendy; Jens Andersen | Zendy; Peter Arctander | Zendy

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Identification of miRNA targets with stable isotope labeling by amino acids in cell culture

Author(s) -

Jeppe Vinther,

Mads M. Hedegaard,

Paul P. Gardner,

Jens Andersen,

Peter Arctander

Publication year - 2006

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkl590

Subject(s) - stable isotope labeling by amino acids in cell culture , biology , microrna , proteome , gene , proteomics , hela , computational biology , gene expression , amino acid , transfection , drosha , regulation of gene expression , genetics , microbiology and biotechnology , cell , rna , rna interference

miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell proteome. Expression of 12 out of 504 investigated proteins was repressed by miRNA-1 transfection. This repressed set of genes significantly overlaps with miRNA-1 regulated genes that have been identified with DNA array technology and are predicted by computational methods. Moreover, we find that the 3'-untranslated region for the repressed set are enriched in miRNA-1 complementary sites. Our findings demonstrate that SILAC can be used for miRNA target identification and that one highly expressed miRNA can regulate the levels of many different proteins.

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