Development of a species-specific RNA polymerase I-based shRNA expression vector | Zendy

Maria S. Brenz Verca | Zendy; Peter Weber | Zendy; Christine Mayer | Zendy; C. Graf | Zendy; Damián Refojo | Zendy; Ralf Kühn | Zendy; Ingrid Grummt | Zendy; Beat Lutz | Zendy

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Development of a species-specific RNA polymerase I-based shRNA expression vector

Author(s) -

Maria S. Brenz Verca,

Peter Weber,

Christine Mayer,

C. Graf,

Damián Refojo,

Ralf Kühn,

Ingrid Grummt,

Beat Lutz

Publication year - 2006

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkl1045

Subject(s) - rna polymerase iii , biology , small hairpin rna , rna interference , rna polymerase i , microbiology and biotechnology , trans acting sirna , small interfering rna , rna polymerase ii , transcription (linguistics) , rna induced transcriptional silencing , rna silencing , rna dependent rna polymerase , polymerase , rna , gene expression , promoter , genetics , gene , linguistics , philosophy

RNA interference (RNAi) can be induced in vitro either by application of synthetic short interfering RNAs (siRNAs), or by intracellular expression of siRNAs or short hairpin RNAs (shRNAs) from trans- fected vectors. The most widely used promoters for siRNA/shRNA expression are based on polymerase III (Pol III)-dependent transcription. We developed an alternative vector for siRNA/shRNA expression, using a mouse RNA polymerase I (Pol I) promoter. Pol I-dependent transcription serves in cells for pro- duction of ribosomal RNA (rRNA), and as such, is ubiquitously and stably active in different cell types. As Pol I-dependent transcription is highly species- specific, Pol I-based system provides an important biosafety advantage with respect to silencing of genes with unknown functions.

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