Fast Fenton footprinting: a laboratory-based method for the time-resolved analysis of DNA, RNA and proteins | Zendy

I. Shcherbakova | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Fast Fenton footprinting: a laboratory-based method for the time-resolved analysis of DNA, RNA and proteins

Author(s) -

I. Shcherbakova

Publication year - 2006

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkl055

Subject(s) - footprinting , dna footprinting , dna , rna , deoxyribonuclease i , biology , nucleic acid , macromolecule , biophysics , combinatorial chemistry , biochemistry , chemistry , dna binding protein , base sequence , transcription factor , gene

'Footprinting' describes assays in which ligand binding or structure formation protects polymers such as nucleic acids and proteins from either cleavage or modification; footprinting allows the accessibility of individual residues to be mapped in solution. Equilibrium and time-dependent footprinting links site-specific structural information with thermodynamic and kinetic transitions. The hydroxyl radical (*OH) is a particularly valuable footprinting probe by virtue of it being among the most reactive of chemical oxidants; it reports the solvent accessibility of reactive sites on macromolecules with as fine as a single residue resolution. A novel method of millisecond time-resolved .OH footprinting has been developed based on the Fenton reaction, Fe(II) + H2O2 --> Fe(III) + *OH + OH-. This method can be implemented in laboratories using widely available three-syringe quench flow mixers and inexpensive reagents to study local changes in the solvent accessibility of DNA, RNA and proteins associated with their biological function.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research