NFI and Oct-1 bend the Ad5 origin in the same direction leading to optimal DNA replication | Zendy

M. E. Mysiak | Zendy

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NFI and Oct-1 bend the Ad5 origin in the same direction leading to optimal DNA replication

Author(s) -

M. E. Mysiak

Publication year - 2004

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkh944

Subject(s) - biology , dna replication , rolling circle replication , dna , microbiology and biotechnology , histone octamer , dna binding protein , replication protein a , origin of replication , transcription preinitiation complex , biophysics , transcription factor , genetics , promoter , gene , nucleosome , chromatin , gene expression

Two cellular transcription factors, nuclear factor I (NFI) and octamer binding protein (Oct-1), bind simultaneously to their recognition sequences in the Ad5 origin of replication thereby enhancing initiation. Using scanning force microscopy we have previously shown that NFI induces a 60 degrees bend in the origin DNA. Here we demonstrate that Oct-1 induces a 42 degrees bend in the origin DNA. Simultaneous binding of NFI and Oct-1 induces an 82 degrees collective bend suggesting that both bends are oriented towards each other. In functional replication assays we further demonstrate that this extensive DNA bending leads to a synergistic enhancement of DNA replication. We propose that collective DNA bending induced by NFI and Oct-1 facilitates the optimal assembly of the preinitiation complex and plays an important role in the stimulatory mechanism of NFI and Oct-1 in replication.

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