Transcriptional control of SV40 T-antigen expression allows a complete reversion of immortalization | Zendy

Tobias May | Zendy

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Transcriptional control of SV40 T-antigen expression allows a complete reversion of immortalization

Author(s) -

Tobias May

Publication year - 2004

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkh887

Subject(s) - biology , reversion , sv40 large t antigen , antigen , phenotype , gene expression , gene , microbiology and biotechnology , cell cycle , regulation of gene expression , vector (molecular biology) , embryo , genetics , transfection , recombinant dna

Conditional proliferation of mouse embryo fibroblasts was achieved with a novel autoregulatory vector for Tet-dependent expression of the SV40 T-antigen. The majority of cell clones that were isolated under induced conditions showed strict regulation of cell growth. Status switches were found to be fully reversible and highly reproducible with respect to gene expression characteristics. A consequence of T-antigen expression is a significant deregulation of >400 genes. Deinduced cells turn to rest in G0/G1 phase and exhibit a senescent phenotype. The cells are not oncogenic and no evidence for transformation was found after several months of cultivation. Conditional immortalization allows diverse studies including those on cellular activities without the influence of the immortalizing gene(s), senescence as well as secondary effects from T-antigen expression.

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