Lack of homologous sequence-specific DNA methylation in response to stable dsRNA expression in mouse oocytes | Zendy

Petr Svoboda | Zendy

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Lack of homologous sequence-specific DNA methylation in response to stable dsRNA expression in mouse oocytes

Author(s) -

Petr Svoboda

Publication year - 2004

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkh697

Subject(s) - biology , rna silencing , rna interference , dna methylation , gene silencing , microbiology and biotechnology , trans acting sirna , rna directed dna methylation , gene knockdown , rna , bisulfite sequencing , methylation , cpg site , epigenetics of physical exercise , rna induced transcriptional silencing , genetics , dna , gene , gene expression

Double-stranded RNA (dsRNA) induces sequence-specific mRNA degradation in most eukaryotic organisms via a conserved pathway known as RNA interference (RNAi). Post-transcriptional gene silencing by RNAi is also connected with transcriptional silencing of cognate sequences. In plants, this transcriptional silencing is associated with sequence-specific DNA methylation. To address whether this mechanism operates in mammalian cells, we used bisulfite sequencing to analyze DNA in mouse oocytes constitutively expressing long dsRNA against the Mos gene. Our data show that long dsRNA induces efficient Mos mRNA knockdown but not CpG and non-CpG DNA methylation of the endogenous Mos sequence in oocytes and early embryos. These data demonstrate that dsRNA does not directly induce DNA methylation in the trans form of this sequence in these mammalian cells.

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