TROD: T7 RNAi Oligo Designer | Zendy

Peter Dudek | Zendy; Didier Picard | Zendy

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TROD: T7 RNAi Oligo Designer

Author(s) -

Peter Dudek,

Didier Picard

Publication year - 2004

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkh360

Subject(s) - rna interference , biology , t7 rna polymerase , computational biology , oligonucleotide , rna , small interfering rna , genetics , dna , rna polymerase , gene , microbiology and biotechnology , escherichia coli , bacteriophage

We have developed T7 RNAi Oligo Designer (TROD), a web application for RNA interference studies. TROD greatly facilitates the design of oligodeoxynucleotide sequences for the in vitro production of siRNA duplexes with T7 RNA polymerase. Given a query cDNA sequence, the program scans for appropriate target sequences based on the constraints of the T7 RNA polymerase method and published criteria for RNA interference with siRNAs. The output is an ordered and prioritized list of ready-to-order DNA oligonucleotide sequences, with links to perform a BLAST search to ascertain target sequence specificity. The TROD web service is available at http://www.cellbio.unige.ch/RNAi.html.

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