The vertebrate protein CTCF functions as an insulator in Saccharomyces cerevisiae | Zendy

PierreAntoine Defossez | Zendy

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The vertebrate protein CTCF functions as an insulator in Saccharomyces cerevisiae

Author(s) -

PierreAntoine Defossez

Publication year - 2002

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkf629

Subject(s) - ctcf , biology , saccharomyces cerevisiae , insulator (electricity) , enhancer , chromatin , genetics , gene , protein domain , computational biology , microbiology and biotechnology , transcription factor , electrical engineering , engineering

Insulators are elements that shelter genes from the effects of silencers or enhancers. CTCF is the only vertebrate protein that has a recognized role in transcriptional insulation, but how it exerts its effect is unknown. In an attempt to better understand how CTCF functions, we have used an insulation assay in Saccharomyces cerevisiae. We show that CTCF acts as an insulator in yeast, where it can efficiently block the spreading of repressive telomeric chromatin. We identify two domains of the protein that are responsible for this activity: a short and very potent N-terminal domain, as well as the C-terminus of the protein.

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