Open AccessRobust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs
Author(s) -
Д. М. Бубнов,
Tigran V. Yuzbashev,
Andrey A Khozov,
O. E. Melkina,
Tatiana V. Vybornaya,
GuyBart Stan,
S. P. Sineoky
Publication year - 2022
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/gkac649
Subject(s) - recombineering , biology , genetics , synthetic biology , genome , genome engineering , computational biology , bacterial artificial chromosome , dna , operon , plasmid , genome editing , escherichia coli , gene
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