Flow cytometry assay for the detection of single-copy DNA in human lymphocytes | Zendy

Naoki Uno | Zendy; Norihito Kaku | Zendy; Yoshitomo Morinaga | Zendy; Hiroo Hasegawa | Zendy; Katsunori Yanagihara | Zendy

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Flow cytometry assay for the detection of single-copy DNA in human lymphocytes

Author(s) -

Naoki Uno,

Norihito Kaku,

Yoshitomo Morinaga,

Hiroo Hasegawa,

Katsunori Yanagihara

Publication year - 2020

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/gkaa515

Subject(s) - biology , flow cytometry , microbiology and biotechnology , dna , in situ , nucleic acid , genomic dna , hybridization probe , fluorescence in situ hybridization , in situ hybridization , nucleic acid thermodynamics , genetics , gene , chromosome , gene expression , base sequence , physics , meteorology

Specific nucleic acid sequences can be detected in individual cells by in situ hybridization. However, when very few copies of a target sequence are present per cell, its signal is undetectable by flow cytometry. Although various approaches have been developed to increase fluorescence signals for in situ hybridization, flow cytometric detection of specific genomic DNA sequences has not been established. Here, we present a flow cytometry assay for detection of single-copy genomic sequences in human lymphocytes using in situ PCR with universal energy transfer-labelled primers.

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