The atp operon: nucleotide sequence of the region encoding the α-subunit of Escherichia coli ATP-synthase | Zendy

N Gay | Zendy; John E. Walker | Zendy

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The atp operon: nucleotide sequence of the region encoding the α-subunit of Escherichia coli ATP-synthase

Author(s) -

N Gay,

John E. Walker

Publication year - 1981

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/9.9.2187

Subject(s) - biology , operon , escherichia coli , nucleic acid sequence , nucleotide , microbiology and biotechnology , protein subunit , tryptophan synthase , atp synthase , molecular cloning , peptide sequence , biochemistry , genetics , dna , gene

Part of the atp (or unc) operon encoding the alpha, beta, gamma, delta, and epsilon subunits of Escherichia coli ATP-synthase has been cloned into the plasmid pACYC 184. The DNA coding for the largest of these proteins, the alphas subunit, has been sequenced by cloning into the bacteriophage M13 and sequencing with dideoxy nucleotide chain terminators. It comprises 1539 nucleotides corresponding to a protein of 513 amino acids.

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