Conformational analysis of a modified ribotetranucleoside triphosphate: m62A-U-m62A-U studied in aqueous solution by nuclear magnetic resonance at 500 MHz1 | Zendy

Arnold J. Hartel | Zendy; Gerrie Wille-Hazeleger | Zendy; Jacques H. van Boom | Zendy; C. Altona | Zendy

Open Access

Conformational analysis of a modified ribotetranucleoside triphosphate: m⁶₂A-U-m⁶₂A-U studied in aqueous solution by nuclear magnetic resonance at 500 MHz¹

Author(s) -

Arnold J. Hartel,

Gerrie Wille-Hazeleger,

Jacques H. van Boom,

C. Altona

Publication year - 1981

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/9.6.1405

Subject(s) - tetramer , crystallography , nuclear magnetic resonance spectroscopy , stacking , aqueous solution , chemical shift , decoupling (probability) , stereochemistry , physics , nuclear magnetic resonance , chemistry , enzyme , control engineering , engineering

The complete and unequivocal assignment of the 24 ribose proton signals of m6(2)A(1)-U(2)-m6(2)(3)-U(4) by means of 500 MHz NMR spectroscopy at 17 degrees C is given. this assignment is based on scrupulous decoupling experiments carries out at various temperatures. Analysis of the observed chemical shifts and coupling constants of the tetramer shows that the two fragments -m6(2)A(3)-U(4) comprising the 3'-end occur mainly in the classical right-handed stack conformation, whereas the 5'-end the -U(2)- residue appears bulged out in favour of a less well-defined stacking interaction between the bases m6(2)A(1)-and -m6(2)A(3)-. Conformational populations about each of the torsional degrees of freedom along the backbone are discussed. A modernized version of pseudorotation analysis is used to delineate the conformational behaviour of the four ribose rings.

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