Characterisation of the genes for ribosomal RNA in flax | Zendy

Peter B. Goldsbrough | Zendy; Christopher A. Cullis | Zendy

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Characterisation of the genes for ribosomal RNA in flax

Author(s) -

Peter B. Goldsbrough,

Christopher A. Cullis

Publication year - 1981

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/9.6.1301

Subject(s) - biology , ribosomal rna , tandem repeat , genetics , linum , gene , restriction enzyme , 18s ribosomal rna , coding region , ribosomal dna , plasmid , dna , rna , restriction site , 5s ribosomal rna , restriction fragment , microbiology and biotechnology , phylogenetics , botany , genome

DNA coding for the 18S and 25S rRNAs in flax (Linum usitatissimum) has been purified and is arranged in tandem arrays with a repeat length of 8.6 kb. There is no detectable variation in the size of this repeat unit. Single repeat units have been cloned in the plasmid pAT 153. The coding sequences for the 25S, 18s and 5.85 rRNAs have been localised by hybridisation. The cloned rDNA has been used to compare two genotrophs, L1 ad S1, where the number of rRNA cistrons has been altered by growth under different environmental conditions. In terms of the size of the repeat unit and the position of a number of restriction enzyme sites the rDNAs from L1 and S1 were identical.

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