Molecular cloning and analysis of cDNA sequences derived from polyA+RNA from barley endosperm: identification of B hordein related clones | Zendy

Brian Forde | Zendy; Martin E. Kreis | Zendy; M. Bahman Bahramian | Zendy; Jayne A. Matthews | Zendy; B. J. Miflin | Zendy; Richard D. Thompson | Zendy; Dorothea Bartels | Zendy; R. B. Flavell | Zendy

Open Access

Molecular cloning and analysis of cDNA sequences derived from polyA+RNA from barley endosperm: identification of B hordein related clones

Author(s) -

Brian Forde,

Martin E. Kreis,

M. Bahman Bahramian,

Jayne A. Matthews,

B. J. Miflin,

Richard D. Thompson,

Dorothea Bartels,

R. B. Flavell

Publication year - 1981

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/9.24.6689

Subject(s) - biology , hordein , endosperm , cloning (programming) , complementary dna , genetics , identification (biology) , molecular cloning , rna , dna sequencing , computational biology , microbiology and biotechnology , gene , botany , storage protein , computer science , programming language

A collection of over 130 cDNA clones has been constructed in the bacterial plasmids pPH207 and pBR322 using as template the poly A+ RNA from membrane-bound polysomes of barley endosperm (cv. Sundance). Fifty four B hordein cDNA clones have been identified by cross-hybridization analysis and in vitro translation of plasmid-selected mRNAs. Hybridization of 11 of the B hordein cDNA clones to Northern blots of size-fractionated RNA indicated that the B hordein mRNA is ca. 1300 nucleotides long. One cDNA clone, pHvE-c16, has been partially sequenced and shown by comparison with C-terminal and other peptide sequences to be related to B1 hordein polypeptides. The results obtained from the analysis of the B hordein cDNA clones support the idea that the Hor 2 locus, which specifies the B hordeins, is complex and codes for a family of related mRNA species.

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