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LTK-cells have been transformed to the TK+ phenotype by treatment with size-defined concatamers of HSV-1 TK DNA and Ad2 Bam H1 C fragment (42.0 - 59.5 map units). All TK+ transformants contained Ad2 DNA as well as HSV-1 TK sequences. In most cases several inserts of virus DNA were present, many in high copy numbers. Although no Ad2 transcription promoter was present in the transforming DNA, Ad2-specific sequences were detected in polyadenylated cytoplasmic RNA species from several cell lines.

Adenovirus late sequences linked to herpes simplex virus thymidine kinase may be introduced into eukaryotic cells and transcribed