The use of synthetic oligodeoxynucleotide primers in cloning and sequencing segment 8 of influenza virus (A/PR/8/34) | Zendy

Greg Winter | Zendy; Stan Fields | Zendy; Michael J. Gait | Zendy; George G. Brownlee | Zendy

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The use of synthetic oligodeoxynucleotide primers in cloning and sequencing segment 8 of influenza virus (A/PR/8/34)

Author(s) -

Greg Winter,

Stan Fields,

Michael J. Gait,

George G. Brownlee

Publication year - 1981

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/9.2.237

Subject(s) - biology , cloning (programming) , gene , molecular cloning , virus , bacteriophage , virology , dna , strain (injury) , nucleic acid sequence , microbiology and biotechnology , influenza a virus , dna sequencing , genetics , peptide sequence , escherichia coli , anatomy , computer science , programming language

Complete double-stranded DNA copies of the RNA genes of the human influenza virus A/PR/8/34 have been synthesized by using two synthetic oligodeoxynucleotide primers. The gene encoding the non-structural proteins NS1 and NS2, prepared with these primers, has been cloned into the bacteriophage M13mp7 and sequenced. The sequence is compared with that from another human strain and from an avian strain.

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