Open AccessThe nucleotide sequence of the replication origin of plasmid NTP1
Author(s) -
June N. Grindley,
Dai Nakada
Publication year - 1981
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/9.17.4355
Subject(s) - biology , cole1 , plasmid , genetics , homology (biology) , nucleic acid sequence , base pair , pbr322 , origin of replication , conserved sequence , sequence logo , dna , sequence (biology) , inverted repeat , peptide sequence , autonomously replicating sequence , microbiology and biotechnology , consensus sequence , base sequence , amino acid , gene , genome
The sequence of the DNA of the origin region of NTP1 has been obtained. Analysis of the sequence indicates that: (1) there is great sequence homology in the DNA upstream from the origin in NTP1, ColE1, CLODF13, PBR345 AND PBR322; (2) only seven base pairs of NTP1 are identical with the sequence downstream from the origin in ColE1, although some homology exists for 140 bases downstream; (3) two ten base pair direct repeats are present in NTP1 which are also conserved in all four plasmids named above; (4) probably no polypeptide greater than fifteen amino acids in length is encoded by the NTP1 origin region, since no single open reading frame is conserved in all five plasmids.