Electron microscopic mapping of wheat germ RNA polymerase II binding sites on cloned CaMV DNA | Zendy

Françoise Grellet | Zendy; Richard Cooke | Zendy; Marcel Teissère | Zendy; Michel Delseny | Zendy; Jean Xech | Zendy; Paul Pe | Zendy

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Electron microscopic mapping of wheat germ RNA polymerase II binding sites on cloned CaMV DNA

Author(s) -

Françoise Grellet,

Richard Cooke,

Marcel Teissère,

Michel Delseny,

Jean Xech,

Paul Pe

Publication year - 1981

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/9.16.3927

Subject(s) - biology , genome , dna , polymerase , microbiology and biotechnology , genetics , rna , wheat germ , rna polymerase , gene , biochemistry

The binding sites of wheat germ RNA polymerase II were mapped on the cloned CaMV genome by observation of enzyme-linear DNA complexes by electron microscopy. Twelve sites are observed. Three of them are relatively stable in the presence of heparin and are found at positions 8-9, 21-23, and 41-44 map units on the physical map of the genome. These positions correspond to AT-rich regions of the viral genome which contain potential promoter sites. These results are discussed with reference to current information on the structure and expression of the CaMV genome.

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