Yeast ochre suppressor SUQ5-ol is an altered tRNAUCASer | Zendy

Clive Waldron | Zendy; B. S. Cox | Zendy; Norma M. Wills | Zendy; R.F. Gesteland | Zendy; Peter W. Piper | Zendy; Diane S. Colby | Zendy; C Guthrie | Zendy

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Yeast ochre suppressor SUQ5-ol is an altered tRNA_UCA^Ser

Author(s) -

Clive Waldron,

B. S. Cox,

Norma M. Wills,

R.F. Gesteland,

Peter W. Piper,

Diane S. Colby,

C Guthrie

Publication year - 1981

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/9.13.3077

Subject(s) - transfer rna , biology , suppressor , uridine , genetics , mutant , rna , wobble base pair , microbiology and biotechnology , gene

Ochre suppressor tRNA was partially purified from strains of Saccharomyces cerevisiae containing the serine-inserting class III suppressor SUQ5-ol. RNA sequence analysis of this tRNA indicated that the suppressor is derived from a UCA-decoding tRNA Ser by a G leads to U substitution in the middle position of the anticodon. The suppressor further differs from the wild-type UCA-decoding tRNA Ser in that the mutant anticodon lacks the modified uridine found in the wobble position of the wild-type tRNA and contains instead another modification in or near the anticodon.

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