Characterization of a plasmid-specified ribosome methylase associated with macrolide resistance | Zendy

A G Shivakumar | Zendy; David Dubnau | Zendy

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Characterization of a plasmid-specified ribosome methylase associated with macrolide resistance

Author(s) -

A G Shivakumar,

David Dubnau

Publication year - 1981

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/9.11.2549

Subject(s) - biology , ribosome , plasmid , ribosomal rna , mutant , methyltransferase , gene , transfer rna , erythromycin , microbiology and biotechnology , rna , biochemistry , antibiotics , methylation

The ermC gene of plasmid pE194 specifies resistance to the macrolidelincosamide-streptogramin B antibiotics. This resistance, as well as synthesis of the 29,000 dalton protein product of ermC, has been shown to be induced by erythromycin. Weisblum and his colleagues have established that macrolide resistance is associated with a specific dimethylation of adenine in 23 S rRNA. We show that pE194 specifies an RNA methylase that can utilize either 50 S ribosomes or 23 S rRNA as substrates. Synthesis of this methylase is induced by low concentrations of erythromycin, and the enzyme is produced in elevated amounts by strains carrying a high copy number mutant of pE194. The methylase comigrates with the 29K ermC product on polyacrylamide gels. The purification and some properties of this methylase are described.

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