Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae | Zendy

Dieter Gallwitz | Zendy; Rüdiger W. Seidel | Zendy

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Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae

Author(s) -

Dieter Gallwitz,

Rüdiger W. Seidel

Publication year - 1980

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/8.5.1043

Subject(s) - biology , saccharomyces cerevisiae , microbiology and biotechnology , molecular cloning , plasmid , gene , recombinant dna , actin , dna , pbr322 , nucleic acid sequence , genetics , complementary dna

Two overlapping DNA fragments from yeast Saccharomyces cerevisiae containing the actin gene have been inserted into pBR322 and cloned in E.coli. Clones were identified by hybridization to complementary RNA from a plasmid containing a copy of Dictyostelium actin mRNA. One recombinant plasmid obtained (pYA102) contains a 3.93-kb Hindlll fragment, the other (pYA208) a 5.1-kb Pstl fragment, both share a common 2.2-kb fragment harboring part of the actin gene. Cloned yeast actin DNA was identified by R-loop formation and translation of the hybridized actin mRNA and by DNA sequence analysis. Cytoplasmic actin mRNA has been estimated to be about 1250 nucleotides long. There is only one type of the actin gene in S.cerevisiae.

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