Sequences hybridizing to mRNA, oligo(dT) and dsRNA from pre-mRNA are contiguous in the cloned mouse DNA fragments | Zendy

O. N. Tokarskaya | Zendy; N. А. Tchurikov | Zendy; P.L. Ivanor | Zendy; Dmitri A. Kramerov | Zendy; А. П. Рысков | Zendy

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Sequences hybridizing to mRNA, oligo(dT) and dsRNA from pre-mRNA are contiguous in the cloned mouse DNA fragments

Author(s) -

O. N. Tokarskaya,

N. А. Tchurikov,

P.L. Ivanor,

Dmitri A. Kramerov,

А. П. Рысков

Publication year - 1980

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/8.3.425

Subject(s) - biology , microbiology and biotechnology , hindiii , restriction enzyme , complementary dna , haeiii , restriction fragment , nucleic acid thermodynamics , dna , plasmid , pbr322 , southern blot , messenger rna , ecori , restriction map , genetics , gene , restriction fragment length polymorphism , polymerase chain reaction , base sequence

Fragments from the DNA of mouse embryos produced by restriction endonucleases HindIII were cloned in pBR322 plasmid and examined for the ability to hybridize in situ with [32P] labeled cDNA synthesized from the polysomal poly(A)+mRNA template. Several of the selected clones were examined for the presence of specific sequences inside the cloned mouse DNA fragments by the blotting procedure of southern [1]. The data obtained indicate that the majority of the cloned mouse DNA fragments contained sequences hybridizing with cDNA, oligo(dT) and double-stranded regions from pre-mRNA. The results of hybridization experiments and double digestion with HindIII+HaeIII endonucleases provide evidence that these sequences could be contiguous in the given restriction DNA fragments.

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