A cDNA clone from Zea mays endosperm sucrose synthetase mRNA
Author(s) -
Martin Geiser,
HansPeter Döring,
Johannes Wöstemeyer,
Ute Behrens,
E. Tillmann,
Peter Starlinger
Publication year - 1980
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/8.24.6175
Subject(s) - biology , endosperm , complementary dna , microbiology and biotechnology , southern blot , genomic dna , clone (java method) , nucleic acid sequence , dna , gene , genetics
A cDNA clone for maize endosperm sucrose synthetase of 62o nucleotide pairs length was obtained by cloning double stranded DNA obtained from the total maize endosperm poly(A) RNA in pBR322, and identifying the appropriate clone by hybrid-promoter translation. In Southern blotting to genomic BamHI-digested DNA, a single band only of approximately 20 Kb lights up, indicating that the sucrose synthetase gene is unique, or that closely linked copies are located on this DNA fragment.
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