Sequence of 863 nucleotides encompassing the PstI site of the yeast 2 μm plasmid | Zendy

T C Elleman | Zendy; J. Roger Hindley | Zendy

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Sequence of 863 nucleotides encompassing the PstI site of the yeast 2 μm plasmid

Author(s) -

T C Elleman,

J. Roger Hindley

Publication year - 1980

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/8.21.4841

Subject(s) - psti , biology , ecori , plasmid , restriction site , nucleic acid sequence , genetics , restriction map , sequence (biology) , microbiology and biotechnology , sequence analysis , dna , restriction enzyme

The sequence of part of the larger unique region of the yeast 2 micron plasmid cloned in pMB9 has been determined. The sequence extends from the single EcoRI site in this region to the AvaI site and includes the single PstI site and HpaI site. A notable feature of this sequence is the presence of tandem repeats of 124 residues beginning at the HpaI site and extending beyond the AvaI site. The sequence was determined independently by both the Maxam-Gilbert procedure applied to isolated restriction fragments, and by the chain-termination procedure applied to restriction fragments cloned in the single-stranded phage M13mp2 and purified by plaque selection.

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