Open AccessSelective blotting of restriction DNA fragments on nitrocellulose membranes at low salt concentrations
Author(s) -
Yoshikuni Nagamine,
André Sentenac,
P. Fromageot
Publication year - 1980
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/8.11.2453
Subject(s) - nitrocellulose , agarose , biology , membrane , dna , blot , restriction fragment , electroblotting , southern blot , salt (chemistry) , microbiology and biotechnology , biochemistry , restriction enzyme , chromatography , gene , chemistry
The pattern of restriction DNA fragments transferred from agarose gel to nitrocellulose membranes (Southern's technique) can be affected by the salt concentration of the transfer solvent.
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