Size fractionation of DNA fragments by liquid-liquid chromatography | Zendy

Werner Müller | Zendy; Harald Schuetz | Zendy; Cecilia Guerrier-Takada | Zendy; Patricia E. Cole | Zendy; Russell O. Potts | Zendy

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Size fractionation of DNA fragments by liquid-liquid chromatography

Author(s) -

Werner Müller,

Harald Schuetz,

Cecilia Guerrier-Takada,

Patricia E. Cole,

Russell O. Potts

Publication year - 1979

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/7.8.2483

Subject(s) - fractionation , chromatography , dna , restriction enzyme , biology , polyethylene glycol , dextran , cellulose , biochemistry , chemistry

A method for the fractionation of double-stranded DNA fragments from 150 to 22000 b.p. in size by liquid-liquid chromatography is described. The procedure makes use of the fact that the partitioning of DNA in a polyethylene glycol-dextran system is size dependent and can be altered by alkali metal cations. Cellulose or celite are used as supports for the stationary, dextran-rich phase. Examples show the fractionation of digests of T7 DNA produced by Dpn II and Hind II restriction endonulceases as well as lambda DNA digests produced by Hind III and Eco RI restriction endonucleases.

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