Specific chemical labeling of DNA fragments | Zendy

Hilbert Eshaghpour | Zendy; Dieter Söll | Zendy; D M Crothers | Zendy

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Specific chemical labeling of DNA fragments

Author(s) -

Hilbert Eshaghpour,

Dieter Söll,

D M Crothers

Publication year - 1979

Publication title -

nucleic acids research

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/7.6.1485

Subject(s) - biology , dna , biochemistry , chemical modification , microbiology and biotechnology , molecule , chemistry , organic chemistry

We describe a simple method for specific chemical labeling of DNA fragments at their 3'-termini. The procedure includes enzymatic addition of 4-thiouridine, followed by reaction in mild non-denaturing conditions with the highly reactive alpha-haloacetamido derivatives of several chemical labels. The attached reporter molecule can be removed by extended treatment with beta-mercaptoethanol. Among the potential applications of this labeling method is the study of specific protein-DNA interactions in solution.

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