Isolation and characterization of cloned human fetal globin genes | Zendy

Francesco Ramirez | Zendy; A. Lee Burns | Zendy; J. Gregory Mears | Zendy; Sally E. Spence | Zendy; D Starkman | Zendy; Arthur Bank | Zendy

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Isolation and characterization of cloned human fetal globin genes

Author(s) -

Francesco Ramirez,

A. Lee Burns,

J. Gregory Mears,

Sally E. Spence,

D Starkman,

Arthur Bank

Publication year - 1979

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/7.5.1147

Subject(s) - biology , gene , insert (composites) , genetics , microbiology and biotechnology , clone (java method) , dna , molecular cloning , cloning (programming) , restriction map , genomic library , restriction fragment , library , nucleic acid sequence , peptide sequence , mechanical engineering , computer science , engineering , programming language , 16s ribosomal rna

Three clones containing both the human G gamma and A gamma globlin genes have been isolated and characterized from a library of DNA fragments generated by partial Eco RI digestion of cellular DNA using charon 4A phage as vector. Two of the clones (NY 2 and 3) are identical and have an insert of 14.0 kb. The third clone (NY 1) has a 15.4 kb insert by virtue of an extra 1.4 kb Eco RI fragment at its 5' most end. This clone also has a Kpn I site not present in the other two suggesting it is the product of the gamma gene on the opposite chromosome. Restriction analysis of the three clones indicates that the G gamma and A gamma genes are linked on a single continuous piece of DNA and are separated by 3.5 kb and each contains at least one large intervening sequence of 0.85 kg between the Bam HI and Eco RI sites. These findings in cloned DNA provide direct evidence for linkage and organization of the gamma genes in man.

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