Sequence of 1019 nucleotides encompassing one of the inverted repeats from the yeast 2 μm plasmid | Zendy

J. Roger Hindley | Zendy; Geraldine Phear | Zendy

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Sequence of 1019 nucleotides encompassing one of the inverted repeats from the yeast 2 μm plasmid

Author(s) -

J. Roger Hindley,

Geraldine Phear

Publication year - 1979

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/7.2.361

Subject(s) - inverted repeat , biology , klenow fragment , direct repeat , genetics , nucleic acid sequence , palindrome , nucleotide , plasmid , dna polymerase i , base pair , microbiology and biotechnology , dna , polymerase , polymerase chain reaction , base sequence , gene , exonuclease , reverse transcriptase , crispr , genome

A sequence of 1019 nucleotides encompassing one of the 600 base inverted repeats and non-repeated flanking regions has been determined in the type A yeast 2 micrometers plasmid cloned in pMB9. Methods are described for applying the Maxam-Gilbert sequencing procedure to DNA fragments labelled at the 3'-end using a T4-polymerase exchange/repair reaction and for sequencing 5'-end labelled fragments using dideoxy-nucleotides as chain terminators in the presence of E. coli DNA polymerase (nach Klenow). A notable feature of the sequence is its unusual content of symmetry elements. In one region of 140 nucleotides, 137 are involved in a complex arrangement of direct and inverted repeats linked by palindromic sequences.

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