Interaction of a limited set of proteins with different mRNAs and protection of 5′-caps against pyrophosphatase digestion in initiation complexes | Zendy

Nahum Sonenberg | Zendy; Maureen A. Morgan | Zendy; Douglas Testa | Zendy; Richard J. Colonno | Zendy; Aaron J. Shatkin | Zendy

Open Access

Interaction of a limited set of proteins with different mRNAs and protection of 5′-caps against pyrophosphatase digestion in initiation complexes

Author(s) -

Nahum Sonenberg,

Maureen A. Morgan,

Douglas Testa,

Richard J. Colonno,

Aaron J. Shatkin

Publication year - 1979

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/7.1.15

Subject(s) - biology , messenger rna , rnase p , biochemistry , digestion (alchemy) , microbiology and biotechnology , eukaryotic initiation factor , translation (biology) , protein biosynthesis , rna , gene , chemistry , chromatography

A variety of 5'-3H-methyl-labeled, oxidized viral mRNAs were used as probes for detecting in wheat germ initiation complexes proteins that interact with, and can be cross-linked to, the 5'-cap structure. A limited and reproducible set of specific proteins was obtained with the different mRNAs. The binding of these proteins to the 5'-end of mRNA apparently results in protection against nucleotide pyrophosphatase digestion of the cap even in initiation complexes in which the 5'-end is susceptible to pancreatic RNase digestion. Cross-linked proteins from mammalian initiation complexes comigrated with several of the subunits of similarly treated eIF-3. A model for cap binding protein interaction with mRNA cap during initiation of translation is suggested.

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