Cloning of human satellite III DNA: different components are on different chromosomes
Author(s) -
Howard J. Cooke,
J. Roger Hindley
Publication year - 1979
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/6.10.3177
Subject(s) - biology , satellite dna , cloning (programming) , dna , satellite , fragment (logic) , microbiology and biotechnology , chromosome , molecular cloning , genetics , gene , peptide sequence , physics , computer science , programming language , astronomy
Two fragments cloned from purified human satellite III DNA do not cross-react with each other. One fragment, for which a partial sequence is reported, hybridises to satellite II as well as III and is shown to originate on chromosome 1. The other cloned fragment originates from the Y chromosome. This fragment has undergone considerable changes in size when cloned in lambda gt WES lambda B. Human satellite III is shown to consist of a number of non-cross-reacting sequences which nevertheless are related by the presence of closely spaced Hin F1 sites.
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