Open AccessExcretion of newly synthesized DNA by tonsil lymphocytes
Author(s) -
Mária Staub,
F. Antoni
Publication year - 1978
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/5.8.3071
Subject(s) - biology , dna , thymidine , dna synthesis , in vitro , tonsil , microbiology and biotechnology , excretion , biochemistry , somatic cell , immunology , gene
The main part of newly synthesized DNA is preferentially released in vitro both by non-stimulated or phytohemagglutinin-stimulated tonsil lymphocytes. Freshly isolated cells excrete DNA faster than phytohemagglutinin-stimulated ones. The acid-precipitable 3H-thymidine lost can be accounted for in the culture medium as labeled, double stranded DNA isolated on hydroxyapatite column.
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