An octamer of histones H3 and 114 forms a compact complex with DNA of nucleosome size | Zendy

Richard H. Simon | Zendy; R. Daniel CameriniOtero | Zendy; Gary Felsenfeld | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

An octamer of histones H3 and 114 forms a compact complex with DNA of nucleosome size

Author(s) -

Richard H. Simon,

R. Daniel CameriniOtero,

Gary Felsenfeld

Publication year - 1978

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/5.12.4805

Subject(s) - histone octamer , nucleosome , biology , sedimentation coefficient , dna , histone , tetramer , biophysics , molecule , histone h1 , crystallography , biochemistry , physics , enzyme , chemistry , quantum mechanics

Equimolar mixtures of histones H3 and H4 have been reconstituted onto DNA of nucleosome core size. Two distinct complexes are formed in a relative abundance that depends on the starting ratio of H3 + H4 to DNA. One of these complexes contains two H3-H4 dimers for each DNA molecule, and has a sedimentation coefficient of 7.5S. The other complex contains an octamer consisting of four H3-H4 dimers, and has a sedimentation coefficient of 10.4S. On the basis of these measurements, we conclude that the octamer complex (but not the tetramer complex) is a fully folded, compact structure resembling the nucleosome.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research